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How to determine the protein concentration with the BCA Protein Assay

Written By Henrik's Lab on Monday, Feb 28, 2022 | 11:00 AM

 
A lot of assays are used in the lab to determine the concentration of protein in a sample. One of the most commonly used techniques to quantify the protein concentration is the BCA assay. References: BCA molecule: https://en.wikipedia.org/wiki/Bicinchoninic_acid#/media/File:Structure_of_bicinchoninic_acid.png https://creativecommons.org/licenses/by-sa/3.0/ https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/chemistry-protein-assays.html https://www.citeqbiologics.com/difference-between-bca-bradford-protein-assays/ Support: SUBSCRIBE: https://www.youtube.com/channel/UCLwhUgAMJwi9nEoM1Stjt5A?sub_confirmation=1 TWITTER: @HenriksLab https://twitter.com/HenriksLab LINKED IN: https://www.linkedin.com/in/henrik-ellinghaus-7326021b0/ Music: Loopop - Subharmonic Bliss (YouTube Audio Library) https://www.youtube.com/watch?v=gYJwy2C4QQs Thanks for reviewing: Roman D. https://twitter.com/RomanMDoll Time Codes: 0:00 Introduction 0:28 BCA assay vs Bradford method 0:49 BCA protein assay 3:00 Data analysis #BCA #protein #concentration

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