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Creating an SDS-PAGE Molecular Weight Standard Curve in Excel

Written By biochemTV on Monday, Mar 07, 2016 | 03:18 AM

 
In this video, I show you how to measure the migration distance for each lane, and how to use that information in Excel to create a standard curve. From there, we're able to calculate the molecular weight for any protein on our SDS-PAGE gel. 00:24 Measuring migration distance 01:27 Calculations in Excel 02:02 Creating a scatter plot 04:24 Estimating molecular weight of any protein band

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